- Paper Report
- Open Access
Effect of anti-c5a on thymocyte apoptosis
- Richard Brasington1
© Biomed Central Ltd 2001
- Published: 23 January 2001
Lymphocytes, especially immature thymocytes, appear to undergo apoptosis vigorously during sepsis, resulting in a dramatic reduction in thymus weight. Complement activation during sepsis results in elevated levels of C3a and C5a in rats, primates and humans, and in vivo blockade of C5a improves survival of rats and primates with sepsis. This study addresses the effect of C5a blockade on thymocyte apoptosis in an experimental model of sepsis in rats.
In this experimental model of sepsis in rats, measures of thymocyte apoptosis (thymic weight loss, DNA fragmentation, caspase-3, -6, and -9 activation, cytochrome release, and reduction in Bcl-XL levels) were dramatically reduced in rats treated with rabbit anti-rat C5a, compared with these parameters in control rats, which received pre-immune rabbit IgG. There was no difference in expression of BAX or Bcl-2 in the rats with cecal ligation versus sham-operated controls. Sepsis produced an increase in nuclear transcription of NF-?B, which was unaffected by C5a blockade.
Experimental sepsis was induced in male Long-Evans rats by ligation and perforation of the cecum. The rats were then sacrificed at different intervals.