- Paper Report
- Open Access
Binding of anti-DNA antibodies on cell surface calreticulin
- Sylvie Fournel1
© Biomed Central Ltd 2001
- Received: 25 July 2001
- Accepted: 25 July 2001
- Published: 25 July 2001
- Anti-DNA antibodies
Systemic lupus erythematosus (SLE) is characterized by the presence of antibodies directed against nuclear components, including DNA. The pathogenicity of these anti-DNA antibodies in lupus disease is complex and involves several mechanisms, such as binding to extracellular antigens directly, immune complex formation and/or antibody penetration into living cells. The mechanism of anti-DNA antibody penetration seems to implicate binding to cell surface molecules. The authors investigated the mechanisms of antibody penetration and attempted to identify the cell surface molecules involved.
Two anti-DNA antibodies (F14.6 and H9.3) bound to cultured cells and entered the cytoplasm. Antibody F14.6 entered the nucleus. A 50 kDa protein was shown to bind to F14.6 and H9.3 in an affinity matrix. The protein was identified as calreticulin and was present on the surface of a number of cell types. Finally, the authors demonstrated that four different anti-DNA antibodies can bind to calreticulin.
ELISA, confocal microscopy, avidin-agarose affinity matrix, microsequencing