- Paper Report
- Open Access
IL-10 reduces arthritis and adenovirus-mediated inflammation
- Cheryl Smythe1
© Biomed Central Ltd 2001
- Received: 3 July 2001
- Published: 31 July 2001
- Adenovirus-mediated gene therapy
Interleukin (IL)-10 is a regulatory cytokine that has been shown to have both immunostimulatory and immunosuppressive properties. It has also been shown to be effective in the symptomatic treatment of some inflammatory conditions. Several studies have demonstrated that IL-10 plays a key immunoregulatory role in rheumatoid arthritis (RA). This study uses a collagen-induced arthritis (CIA) model of RA to investigate whether prolonged, systemic administration of homologous murine IL-10 using adenoviral gene delivery is effective in treating established arthritis. Furthermore, given that intravenous delivery of adenoviral vectors is known to cause inflammation in the liver, the question of whether IL-10 gene delivery can suppress adenovirus-induced hepatic inflammation is addressed.
Adenovirus-delivered IL-10 (AdIL-10) effectively controlled the progression of articular inflammation, while empty vector had no effect. AdIL-10 administration reduced both in vitro lymphocyte proliferation and interferon-a production, but had no effect on levels of antibodies to type II collagen. Systemic adenovirus administration did induce a mild hepatic inflammation, as judged by morphological analysis of the liver, assessment of overall inflammation and, more specifically, portal and parenchymal inflammation, apoptosis and mitoses. This mild hepatic inflammation was overcome by AdIL-10 treatment in two out of three animals. Significant hepatic inflammation was only observed following injection of adenovirus at levels 100-fold higher than the effective therapeutic dose. AdIL-10 treatment also attenuated the delayed-type hypersensitivity (DTH) response to high doses of adenovirus; however, this immunosuppressive effect occurred only if AdIL-10 was administered before sensitization, rather than between sensitization and challenge, indicating that IL-10 blocks the initial priming of T cells.
Recombinant adenovirus construction, CIA, model, cell proliferation assays, DTH reactions, histological analysis