- Paper Report
- Open Access
Complement factor C5 and gene region encoding Fc?RIIb in CIA
- Frances Hall1
© Biomed Central Ltd 2001
- Received: 28 August 2001
- Accepted: 19 September 2001
- Published: 19 September 2001
- collagen-induced arthritis
Collagen-induced arthrtis (CIA) is a mouse model of inflammatory synovial disease. T cells, B cells and anti-collagen type II antibodies are all involved in the pathogenesis of disease. The I-A locus is critical for susceptibility to CIA, and H-2q and H-2r are susceptibility haplotypes. However, several other loci have also been implicated. In this study, the arthritis-susceptible strain, B10.Q, is used, which exhibits disease incidence ~50%. An H-2q congenic is made on the insulin-dependent diabetes mellitus (IDDM)-suceptible NOD mouse background (NOD.Q). NOD.Q x B10.Q F1 and F2 intercrosses are used to determine whether insulin-dependent diabetes and CIA share susceptibility genes.
The NOD.Q congenic and the NOD.Q x B10.Q F1 generation were resistant to CIA. Although NOD mice spontaneously develop insulin-dependent diabetes, it is important to note that NOD.Q and the intercrossed mice did not.
Approximately 25% of the F2 generation was susceptible to CIA and displayed disease onset and severity comparable with B10.Q.
The absence of measurable serum C5 in the F2 generation generally corresponded with resistance to disease, which could thus be attributed partly to the NOD-derived nonfunctional C5 gene.
A second susceptibility region, Cia9, was identified. This contains a number of candidate genes, including Fc?RIIb. An interaction between the Cia2 and Cia9 loci was apparent; in mice heterozygous for Cia2 (C5), inheritance of the B10-derived Cia9 locus was associated with a lower incidence of CIA.
The IDDM susceptibility loci identified in the NOD mouse do not appear to confer susceptibility to CIA. This implies distinct pathogenetic pathways for IDDM and CIA.
Generation of congenic mouse strains, induction of collagen-induced arthritis, linkage study
Kleinau S, Martinsson P, Heyman B: Induction and suppression of collagen-induced arthritis is dependent on distinct Fcg receptors.
J Exp Med 2000, 191:1611-1616 (PubMed abstract).
Wang Y, Kristan J, Hao L, Lenkoski CS, Shen Y, Matis LA: A role for complement in antibody-mediated inflammation: C5-deficient DBA/1 mice are resistant to CIA.
J Immunol 2000, 164:4340-4347 (PubMed abstract).
Jirholt J, Cook A, Emahazion T, Sundvall M, Jansson L, Nordquist N, Pettersson U, Holmdahl R: Genetic linkage analysis of collagen-induced arthritis in the mouse.
Eur J Immunol 1998, 28:3321-3328 (PubMed abstract).
McIndoe RA, Bohlman B, Chi E, Schuster E, Lindhardt M, Hood L: Localization of non-MHC collagen-induced arthritis susceptibility loci in DBA/1j mice.
Proc Natl Acad Sci USA 1999, 96:2210-2214) (PubMed abstract).
- Johansson ACM , Sundler M, Kjellen P, Johannesson M, Cook A, Lindquist A-KB, Nakken B, Bolstad AI, Jonsson R, Alarcon-Riquelme M, Holmdahl R: Genetic control of collagen-induced arthritis in a cross with NOD and C57BL/10 mice is dependent on gene regions encoding complement factor 5 and Fc?RIIb and is not associated with loci controlling diabetes. Eur J Immunol. 2001, 31: 1847-1856.View ArticleGoogle Scholar