- Paper Report
- Open Access
SmD1 83-119 peptide accelerates disease in lupus prone mice
- Fanny Monneaux1
© Biomed Central Ltd 2001
- Received: 29 October 2001
- Accepted: 9 November 2001
- Published: 12 November 2001
- Anti-dsDNA antibodies
- lupus mice
- SmD1 peptides
- systemic lupus erythematosus
Among antibodies found in sera from patients with systemic lupus erythematosus (SLE), those directed against DNA and Sm proteins are specific and have been included as one of the American College of Rheumatology classification criteria for SLE. The authors have previously identified a polypeptide (83-119) of SmD1 protein recognized by 70% of SLE patients' sera. They observed that reactivity to 83-119 polypeptide is higher in anti-dsDNA antibody positive sera than anti-dsDNA antibody negative sera. The present study was undertaken to analyze the influence of the SmD1 83-119 polypeptide on the generation of pathogenic anti-dsDNA antibodies. The authors immunized female lupus-prone prenephritic (NZB x NZW) F1 mice (NZB/NZW mice) with keyhole limpet hemocyanin (KLH)-coupled SmD1 83-119 peptide and measured survival, proteinuria, renal function and anti-dsDNA production.
Immunization of NZB/NZW mice with KLH-SmD1 83-119-but not with control peptide and protein-resulted in decreased survival, increased anti-dsDNA antibody production, enhanced proteinuria and immune complex nephritis, and accelerated production of autoantibodies directed against various self antigens. None of these manifestations were observed in other control mouse strains immunized with KLH-SmD1 83-119. The authors also found that splenocytes from unmanipulated NZB/NZW mice, unlike those from normal mice, proliferated in response to peptide 83-119; this response was further amplified after immunization. Cells from both untreated and treated NZB/NZW mice seldom recognized the recombinant SmD1 protein used as a control.
Immunization, ELISA, immunohistology, T-cell proliferation
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