- Paper Report
- Open Access
Autoreactive marginal B cells in drug-induced lupus
- Sylvie Fournel1
© Biomed Central Ltd 2002
- Received: 30 November 2001
- Accepted: 9 January 2002
- Published: 9 January 2002
- Autoreactive B cells
Expansion of autoreactive B cells could explain the presence of autoantibodies in the autoimmune disease, systemic lupus erythematosus (SLE). To gain better insight into the production of autoreactive B cells, the authors have developed a drug-induced model of lupus in BALB/c mice transgenic for the heavy chain of an anti-DNA antibody. Normally, the transgenic mice display effective regulation of the transgene-expressing anti-DNA B cells. When treated with exogenous 17'-estradiol (E2), autoreactive B cells were activated and mice developed a lupus-like disease.
In this model, only a small population of B cells was activated, showing that the autoimmune disease does not result in polyclonal B-cell activation. However, the splenic B-cell development is altered by E2 treatment, with a decrease in transitional B cells and an increase in marginal zone B cells. Moreover, anti-DNA secreting B cells display a marginal zone B cell phenotype. These alterations do not result from CD4+ T-cell activation.
Flow cytometry, immunochemistry, ELISPOT assay