- Paper Report
- Open Access
Autoantibody detection by arrays
- Martin Aringer1
© Biomed Central Ltd 2002
- Received: 9 April 2002
- Accepted: 10 May 2002
- Published: 10 May 2002
- rheumatoid arthritis
A wide variety of autoantibodies are of diagnostic importance in rheumatic diseases. In addition to this clinical aspect, autoantibody patterns and their development in early disease help improve our understanding of disease pathogenesis. While microarrays are already widely used for nucleic acid screening, a related technique may be very useful for rapidly detecting multiple autoantibodies with small amounts of serum.
With a robotic arrayer, 1152 feature arrays were fabricated that carried 196 distinct autoantigens in replicate sets on poly-L-lysine coated glass slides. These arrays were tested with well-characterized sera of patients with autoimmune diseases, mixtures of these sera and sera of healthy individuals. The autoantibodies were scanned after being made visible with fluorochrome-labeled secondary reagents specific for different immunoglobulin subclasses. By this approach, antibodies to most autoantigens were detectable, including antibodies to post-translational modifications and to linear peptides, but there were some exceptions (e.g. for Sm proteins). The technique was shown to be specific, more sensitive than ELISA, and capable of quantifying autoantibody concentrations. In an alternative approach, with more technical problems due to staining variations, patient and control sera were directly labeled with the fluorochromes Cy3 and Cy5, respectively, and the resulting color (green, red, or yellow for the addition of both) was assayed, as in other arrays.
Roboter-fabricated autoantigen arrays, ELISA
- Robinson WH, DiGennaro C, Hueber W, Haab BB, Kamachi M, Dean EJ, Fournel S, Fong D, Genovese MC, de Vegvar HE, Skriner K, Hirschberg DL, Morris RI, Muller S, Pruijn GJ, van Venrooij WJ, Smolen JS, Brown PO, Steinman L, Utz PJ : Autoantigen microarrays for multiplex characterization of autoantibody responses. Nature Medicine . 2002, 8: 295-301.PubMedView ArticleGoogle Scholar