- Paper Report
Gene expression in regulatory T cells.
Arthritis Research & Therapy volume 4, Article number: 77100 (2002)
Regulatory T cells have been shown to be important in preventing the development of autoimmune disease in animal models. Their existence has been demonstrated in humans, and their role in autoimmune disease and transplantation tolerance is being studied. They are not yet well-defined phenotypically, although they appear to reside within the subset of CD4+ T cells that also express CD25 constitutively. The identification of a unique marker for this population of cells would be a substantial advance. The aim of this research was to identify genes selectively expressed by regulatory T cells in vitro and in vivo, in order to provide markers to monitor the tolerant state and give indications of potential molecular mechanisms.
A range of T cell lines and clones specific for male antigen were generated. These included Th1 and Th2 lines, two in vitro-generated regulatory-type clones, and lines derived from tolerated male skin grafts (tolerance induced by anti-CD4 and CD8 monoclonal antibodies). Naturally occurring mouse spleen CD4+CD25+cells were also purified. RNA was extracted from both resting and activated cells, for subsequent SAGE (series analysis of gene expression) analysis. A proportion of identified markers were then further analysed using flow cytometry or quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
The main candidates from the SAGE data for known genes that may be associated with the regulatory T cell clone Tr1D1 and Tskin cell lines were ppENK, granzyme A, GM2 ganglioside activator protein (Gm2a), cystatin F, integrin ?7, OX40, glucocorticoid-induced TNFR [tumor necrosis factor receptor] superfamily member 18 (GITR), and the cytochrome P450 enzyme Cyp11a. Immunofluorescence demonstrated that a proportion of the resting Treg and Tskin cells were positive for aE integrin (CD103) and that this was maintained after activation. Of note was that 20% of the spleen CD4+CD25+ population expressed aE integrin. This marker has previously been associated with effector cell activity of CD8+ intraepithelial cells in skin and gut, and of note is that aE-deficient mice develop autoimmune-like inflammatory skin lesions when crossed to susceptible backgrounds. Taqman quantitative RT-PCR revealed that ppENK was highly expressed and further upregulated after activation on Treg, Tskin and CD4+CD25+ spleen cells. ppENK is a member of the opioid family and is cleaved to active enkephalin peptides by proteases. It has been reported that met-enkephalin peptides can inhibit induced chemotaxis of TH1 cells through receptor desensitisation, which may suggest a possible mechanism of local Treg action. GM2a was present at high levels in resting Th2, Treg, and Tskin lines and CD4+CD25+ cells but was lost after CD3 stimulation.
This paper demonstrates some similarities between Th2 and Treg cells, although certain Th2-expressed genes are lost (eg. GATA-3 and Egr-1). A number of genes expressed by Th2 cells are further upregulated in Treg clones and Tskin lines, as well as in naturally occurring splenic CD4+CD25+ T cells. Although no specific markers for regulatory T cells were identified, some interesting molecules such as aE integrin were highlighted. These in vivo and in vitrocorrelations begin to suggest potential surrogate markers for the tolerant state.
SAGE, flow cytometry, real-time quantitative RT-PCR.
Zelenika D, Adams E, Humm S, Graca L, Thompson S, Cobbold S, Waldmann H: Regulatory T cells overexpress a subset of Th2 gene transcripts. J of Immunol. 2002, 168(3): 1069-1079.