Figure 3
From: Quantitative biomarker analysis of synovial gene expression by real-time PCR
Coefficient of variation using PCR techniques. (a) The expression of GAPDH was used to compare assay reproducibility utilizing the standard curve and threshold cycle, C(t), methods of analysis. Five assays were performed over the course of 1 week. Note that the percentage coefficient of variation was much greater for the C(t) method compared with that for the standard curve method. (b) The expression of GAPDH was used to compare assay reproducibility utilizing the standard curve or C(t) methods of analysis. Twenty-eight separate assays were performed over 4 months. Note that the percentage coefficient of variation was greater for the C(t) method compared with that for the standard curve method.