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Table 1 Intra-assay variation for quantitative PCR

From: Quantitative biomarker analysis of synovial gene expression by real-time PCR

 

Mean cell equivalents

Standard deviation

Coefficient of variation (%)

GAPDH

237

41

18

IL-1β

1.6

0.2

15

IL-6

11,236

2276

20

MMP-1

4487

1074

24

  1. GAPDH, IL-1β, IL-6 and matrix metalloproteinase 1 (MMP-1) expression were determined in five replicate samples that were each processed separately. Data are reported as cell equivalents of expression relative to the standard. Note: cell equivalents for different targets cannot be compared directly.