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Table 1 Intra-assay variation for quantitative PCR

From: Quantitative biomarker analysis of synovial gene expression by real-time PCR

  Mean cell equivalents Standard deviation Coefficient of variation (%)
GAPDH 237 41 18
IL-1β 1.6 0.2 15
IL-6 11,236 2276 20
MMP-1 4487 1074 24
  1. GAPDH, IL-1β, IL-6 and matrix metalloproteinase 1 (MMP-1) expression were determined in five replicate samples that were each processed separately. Data are reported as cell equivalents of expression relative to the standard. Note: cell equivalents for different targets cannot be compared directly.