Figure 3
From: Patients with systemic lupus erythematosus have abnormally elevated Epstein–Barr virus load in blood
Epstein–Barr virus (EBV) loads in peripheral blood mononuclear cells (PBMCs) from 29 normal individuals and 24 patients with systemic lupus erythematosus (SLE). (a) Sensitivity of PCR/Southern blot for the EBV nuclear antigen (EBNA)-3C sequence. DNA was purified from serial 10-fold dilutions of Namalwa cells (corresponding to 1 to 1 × 107 cells) were mixed with BJAB cells to yield a total cell number of 1 × 107. PCR was performed using a 100th of the purified DNA (corresponding to DNA of 105 cells). The PCR products were separated in an agarose gel, transferred to a membrane, and probed with an EBNA-3C-specific oligonucleotide. (b) EBV loads of normal individuals and SLE patients. The mean EBV load of each group is presented as a heavy horizontal line.