Treatment | Time (h) |
|---|
| | 24 | 48 |
|---|
Diosgenin | 3.1 ± 0.7 | 5.71 ± 1.79 |
Celecoxib + diosgenin | 0.86 ± 0.16* | 0.29 ± 0.08# |
IL-1β + diosgenin | 120.40 ± 17.44* | 792.24 ± 56.33# |
- Human rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) were preincubated with or without celecoxib (1 μM) or IL-1β (1 ng/ml) for 4 hours, and then 40 μM diosgenin was added for 24 or 48 hours. The concentration of prostaglandin E2 (PGE2) was measured by enzyme immunoassay and is expressed asng/ml for 105 cells. Data are PGE2 concentrations expressed as mean ± SD of three experiments from three different patients. The PGE2 concentrations under the other conditions at 24 or 48 hours, respectively, were 1.39 ± 0.41 and 1.21 ± 0.26 for culture with medium alone, 0.73 ± 0.19 and 0.66 ± 0.12 for celecoxib alone, and 20.26 ± 3.19 and 17.42 ± 3.03 for IL-1β alone. * ,#A P value of less than 0.05 (Fisher's protected-least-significant-difference test) was considered to indicate significance in comparison with diosgenin alone.
