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Figure 4 | Arthritis Research & Therapy

Figure 4

From: The effects of 1α,25-dihydroxyvitamin D3 on matrix metalloproteinase and prostaglandin E2 production by cells of the rheumatoid lesion

Figure 4

The effects of 1α,25-hydroxyvitamin D3 (1,25) on prostaglandin E2 (PGE2) production by rheumatoid synovial fibroblasts (RSFs) and human articular chondrocytes (HACs) after 48 h incubation. (a) PGE2production by RSFs (n = 3) showing normalized data for control; + 1,25 (10-8 mol/l); + interleukin (IL)-1β (0.05 ng/ml); and + IL-1β and 1,25 (0.05 ng/ml and 10-8 mol/l, respectively). Before normalization, control values for PGE2 production by RSFs were in the range 500–2000 pg/ml culture medium/106 cells per 48 h. (b) PGE2 production by HACs (n = 3) showing normalized data for control; + 1,25; + IL-1β; and + IL-1β and 1,25. Before normalization control values for PGE2 production by HAC were in the range 100–300 pg/ml culture medium/106 cells per 48 h. (c) Normalized data for PGE2production by IL-1β-stimulated RSFs after preincubation with 1α,25(OH)2D3, as follows: A, control; B, +IL-1β; C, 1,25 + IL-1β; D, 1 h preincubation with 1,25, then + IL-1β and 1,25; E, 16 h preincubation with 1,25, then IL-1β + 1,25; F, 16 h preincubation with 1,25, then IL-1β alone. Values are shown as means ± SEM.

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