Table 3 Antibodies used in the present study
Antibody (technique) | Antigen recognized | Cellular localization | Main cellular expression |
|---|---|---|---|
MOPC 21 (F/I) | - | [IgG1 isotype control] | |
UPC-10 (F/I) | - | [IgG2a isotype control] | |
Rabbit Ig/rabbit serum (F/I) | - | [rabbit Ig control] | |
Monoclonal | |||
AS02 (F/I) | Thy-1 (CD 90) | Membrane | Fibroblasts, endothelial cells, thymocytes |
W6/32 (F) | MHC-I (A, B, C) | Membrane | Ubiquitous |
CII 1.47 (F) | MHC-II (Pan) | Membrane | Antigen presenting cells, fibroblasts |
SJD1 (F) | CD13 | Membrane | Macrophages, fibroblasts, PMN, T cells |
3-2B12 (F/I) | Prolyl-4-hydroxylase | Cytoplasm | Fibroblasts, endothelial cells |
V9 (F) | Vimentin | Cytoplasm | Fibroblasts, endothelial cells |
4F9 (F/I) | von Willebrand factor | Cytoplasm | Endothelial cells |
UCHT-1 (F) | CD3 | Membrane | T cells |
TM316 (F) | CD11b | Membrane | Monocytes/macrophages, PMN, NK cells |
MoS39 (F/I) | CD14 | Membrane | Monocytes/macrophages |
Tyk 4 (F/I) | CD14 | Membrane | Monocytes/macrophages |
80H5 (F) | CD15 | Membrane | PMN, monocytes |
HD 37 (F) | CD19 | Membrane | B cells |
B-Ly 1 (F) | CD20 | Membrane | B cells |
Cadherin 5(F/I) | CD144 | Membrane | Endothelial cells |
AT 13/5 (F) | CD38 | Membrane | Plasma cells, activated T cells |
NKH/1 (F) | CD56 | Membrane | NK cells |
PG-M1 (F/I) | CD68 | Membrane/cytoplasm | Macrophages* |
HB 15a (F) | CD83 | Membrane | Dendritic cells, activated lymphocytes |
BBIG-V1(F/I) | VCAM-1 (CD 106) | Membrane | Fibroblasts, endothelial cells |
1.4C3 (F) | VCAM-1 (CD 106) | Membrane | Fibroblasts, endothelial cells |
Polyclonal | |||
MP I (F/I) | Procollagen I | Cytoplasm | Fibroblasts |
MP III (F/I) | Procollagen III | Cytoplasm | Fibroblasts |
Anti-c-Fos(F) | c-Fos | Nucleus/cytoplasm | Ubiquitous |
Anti-c-Jun(F) | c-Jun | Nucleus/cytoplasm | Ubiquitous |