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Table 3 Antibodies used in the present study

From: Isolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture — primary culture cells markedly differ from fourth-passage cells

Antibody (technique) Antigen recognized Cellular localization Main cellular expression
MOPC 21 (F/I) -   [IgG1 isotype control]
UPC-10 (F/I) -   [IgG2a isotype control]
Rabbit Ig/rabbit serum (F/I) -   [rabbit Ig control]
Monoclonal    
   AS02 (F/I) Thy-1 (CD 90) Membrane Fibroblasts, endothelial cells, thymocytes
   W6/32 (F) MHC-I (A, B, C) Membrane Ubiquitous
   CII 1.47 (F) MHC-II (Pan) Membrane Antigen presenting cells, fibroblasts
   SJD1 (F) CD13 Membrane Macrophages, fibroblasts, PMN, T cells
   3-2B12 (F/I) Prolyl-4-hydroxylase Cytoplasm Fibroblasts, endothelial cells
   V9 (F) Vimentin Cytoplasm Fibroblasts, endothelial cells
   4F9 (F/I) von Willebrand factor Cytoplasm Endothelial cells
   UCHT-1 (F) CD3 Membrane T cells
   TM316 (F) CD11b Membrane Monocytes/macrophages, PMN, NK cells
   MoS39 (F/I) CD14 Membrane Monocytes/macrophages
   Tyk 4 (F/I) CD14 Membrane Monocytes/macrophages
   80H5 (F) CD15 Membrane PMN, monocytes
   HD 37 (F) CD19 Membrane B cells
   B-Ly 1 (F) CD20 Membrane B cells
   Cadherin 5(F/I) CD144 Membrane Endothelial cells
   AT 13/5 (F) CD38 Membrane Plasma cells, activated T cells
   NKH/1 (F) CD56 Membrane NK cells
   PG-M1 (F/I) CD68 Membrane/cytoplasm Macrophages*
   HB 15a (F) CD83 Membrane Dendritic cells, activated lymphocytes
   BBIG-V1(F/I) VCAM-1 (CD 106) Membrane Fibroblasts, endothelial cells
   1.4C3 (F) VCAM-1 (CD 106) Membrane Fibroblasts, endothelial cells
Polyclonal    
   MP I (F/I) Procollagen I Cytoplasm Fibroblasts
   MP III (F/I) Procollagen III Cytoplasm Fibroblasts
   Anti-c-Fos(F) c-Fos Nucleus/cytoplasm Ubiquitous
   Anti-c-Jun(F) c-Jun Nucleus/cytoplasm Ubiquitous
  1. *The only anti-CD68 monoclonal antibody reported to be specific for macrophages (data not shown) [38]. F, Antibody used for flow cytometry (FACS) analysis only; F/I, antibody used for FACS and immunohistochemistry; MHC, major histocompatibility complex; VCAM-1, vascular cell adhesion molecule-1; NK, natural killer.