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Table 5 Phenotype of negatively isolated primary-culture rheumatoid arthritis (RA)-synovial fibroblasts (SFB) in comparison with fourth-passage RA-SFB

From: Isolation and characterization of rheumatoid arthritis synovial fibroblasts from primary culture — primary culture cells markedly differ from fourth-passage cells

  Primary-culture Fourth-passage Fourth-passage
  RA-SFB RA-SFB RA-SFB
  Isolated cells Isolated cells Conventionally passaged cells
  (n = 9 patients) (n = 3 patients) (n = 6 patients)
Antigen Percent MFI Percent MFI Percent MFI
Thy-1 (CD90; mAb AS02) 73.8 ± 9.6 109 ± 20 86.6 ± 4.6 41 ± 7 # 85.9 ± 4.3 95 ± 17 x
MHC-I a77.2 ± 8.1 130 ± 54 99.2 ± 0.2 # 117 ± 48 97.4 ± 1.4 # 175 ± 78
MHC-II a65.8 ± 12.3 223 ± 84 4.7 ± 2.9 # 59 ± 25 4.8 ± 2.4 # 80 ± 21
Aminopeptidase N (CD13) 84.8 ± 5.9 444 ± 226 97.2 ± 1.6 108 ± 49 97.9 ± 1.2 # 236 ± 95
VCAM-1 (CD106) c17.5 ± 9.8 122 ± 42 n.d. n.d. c0.6 ± 0.1 21 ± 5 #
Prolyl-4-hydroxylase 84.8 ± 2.9 164 ± 45 98.7 ± 0.8 # 164 ± 62 98.3 ± 0.7 # 189 ± 82
Vimentin a93.2 ± 1.5 592 ± 290 99.5 ± 0.4 # 717 ± 126 99.7 # 325
Procollagen I a44.8 ± 13.0 25 ± 2 92.7 ± 2.8 # 25 ± 12 91.0 ± 4.0 # 28 ± 5
Procollagen III a50.0 ± 14.3 26 ± 3 94.1 ± 1.8 # 21 ± 12 90.2 ± 5.2 # 22 ± 4
c-Fos a57.4 ± 14.0 20 ± 2 63.5 ± 16.4 10 ± 5 95.0 ± 0.6 #x 17 ± 2
c-Jun b42.5 ± 14.0 21 ± 5 n.d. n.d. d42.1 ± 12.0 9 ± 2 #
Jun-D a53.0 ± 14.8 21 ± 4 68.1 ± 13.2 11 ± 5 93.0 ± 1.3 #x 18 ± 3
  1. Data presented as means ± SEM. MFI, Mean fluorescence intensity; mAb, monoclonal antibody; MHC, major histocompatibility complex; VCAM-1, vascular adhesion molecule-1; n.d., not determined. For vimentin expression in conventionally passaged fourth-passage RA-SFB, the median is shown because of a large deviation from the mean. Significant differences between groups are indicated in bold. #P ≤ 0.05 Mann–Whitney U test versus primary-culture RA-SFB. xP ≤ 0.05 Mann–Whitney U test versus fourth-passage RA-SFB (isolated cells). an = 7 RA patients. bn = 6 RA patients. cn = 5 RA patients. dn = 4 RA patients.