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Figure 1 | Arthritis Res Ther

Figure 1

From: Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4+T cells from patients with rheumatoid arthritis

Figure 1

Dose response of IL-10 inhibition of interferon gamma (IFN-γ) production by CD4+ T cells after CD3 and CD28 costimulation in patients with rheumatoid arthritis (RA) and in healthy controls (HC). CD4+ T cells were purified from peripheral blood mononuclear cells of three RA patients and three HC by positive selection with anti-CD4 antibody. CD4+ T cells (5 × 105 cells in 0.5 ml culture medium with 10% FCS) were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody in the presence or absence of diluted IL-10 concentrations for 36 hours. Culture supernatants were measured for concentrations of IFN-γ by ELISA. IFN-γ production with IL-10 expressed as % IFN-γ production without IL-10. Values are the mean ± standard error of the mean.

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Arthritis Research & Therapy

ISSN: 1478-6362

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