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Figure 5 | Arthritis Res Ther

Figure 5

From: Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor κB-dependent pathway in patients with rheumatoid arthritis

Figure 5

Effects of LY294002 or pyrrolidine dithiocarbamate (PDTC) on anti-CD3 antibody-triggered interleukin (IL)-17 mRNA expression by peripheral blood mononuclear cells (PBMC) from patients with rheumatoid arthritis. PBMC were cultured with medium only (lane 1), anti-CD3 antibody (1 μg/ml; lane 2), anti-CD3 antibody (10 μg/ml; lane 3), anti-CD3 antibody (10 μg/ml) plus LY294002 (20 μM; lane 4) or anti-CD3 antibody (10 μg/ml) plus PDTC (300 μM; lane 5) for 12 hours; lane 6 shows a negative control. Total RNA (2 μg) was used for cDNA synthesis in a volume of 20 μl; 1 μl of the synthesized cDNA was used for reverse transcription–polymerase chain reaction as described. PCR reaction product (25 μl) was separated on an agarose gel containing ethidium bromide. The relative intensities of the bands were revealed under UV radiation.

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Arthritis Research & Therapy

ISSN: 1478-6362

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