Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor κB-dependent pathway in patients with rheumatoid arthritis

Table 1 Production of interleukin-17 in response to anti-CD3 and mitogens by peripheral blood mononuclear cells and T cells from patients with rheumatoid arthritis

Rheumatoid arthritis (RA) peripheral blood mononuclear cells (PBMC) were stimulated for 24 hours with anti-CD3 (10 μg/ml) plus anti-CD28 antibody (1 μg/ml), phytohemagglutinin (PHA; 5 μg/ml), or none of these (medium only). RA T cells were stimulated for 24 hours with anti-CD3 (10 μg/ml) and PHA (5 μg/ml). The levels of interleukin-17 were measured in culture supernatants by enzyme-linked immunosorbent assay. Results are means ± SEM of three independent experiments.

ISSN: 1478-6362