Figure 7

Monocyte migration induced by supernatants of RA SCLs stimulated with TNF-α. Assay of monocyte migration was performed using supernatants of RA SCLs stimulated with TNF-α. Each RA SCL (1 × 10⁵/well) was cultured in six-well culture plates with 2 ml DMEM supplemented with 5% FBS with or without 2 ng/ml TNF-α. Supernatants were collected after 24 h of incubation, and were used for assay. Migrated cells were stained with anti-CD14 mAb, then the number of migrated CD14⁺ cells was assessed by flow cytometry. RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from three independent experiments. Statistical analysis was performed with the paired Student t test. ^*P < 0.05 and ^**P < 0.01.