Phenotypic characteristics of human monocytes undergoing transendothelial migration

Table 2 Surface marker expression on different monocyte populations

Marker	Initial	NAD	BND	MIG
CD11a	312 ± 138	301 ± 154^‡	273 ± 150	362 ± 187^*†
CD33	138 ± 63	130 ± 49^‡	118 ± 49	148 ± 70^*†
CD45RA	24 ± 15	25 ± 11	27 ± 11	46 ± 47
CD45RB	397 ± 149	367 ± 135‡	326 ± 134	375 ± 154^†
CD45RO	64 ± 35	80 ± 44	79 ± 50	111 ± 57^*†
CD49d	56 ± 10	53 ± 10	53 ± 12	65 ± 28
CD54	41 ± 32	36 ± 12	35 ± 15	58 ± 33^*†
CD62L	22 ± 24	30 ± 24	29 ± 26	44 ± 33^†
CD86	26 ± 19	36 ± 30	66 ± 103	26 ± 14
HLA-DR	174 ± 83	186 ± 89	207 ± 104	326 ± 254^*†

Data shown are fluorescence intensities (means ± SD) of the initial population and the nonadherent (NAD), bound (BND) and migrated (MIG) monocytes. In all 14 experiments performed, the migration period was 30 minutes; the endothelium was not pretreated. ^*Statistical significance (P < 0.05) MIG compared with NAD. ^†Statistical significance (P < 0.05) MIG compared with BND. ^‡Statistical significance (P < 0.05) NAD compared with BND.

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