Figure 3

Hyaluronan (HA) substrate gel electrophoresis of fibroblast cell layers extracted with octylglucoside. After electrophoresis, gels were incubated at 37°C for 16 hours at pH 4 or pH 6.5 before being treated with proteinase K and stained with alcian blue. Lane 1, fibroblast cell layers extracted with octylglucoside; lane 2, a preparation of liver lysosomes; lane 3, a commercial preparation of bovine testicular hyaluronidase. Standards were Precision Plus Protein standards from Bio-Rad, with major bands at 75 and 50 kDa).