Table 2 Real-time PCR probes and details of primers
From: The role of interleukin-1 in the pathogenesis of human Intervertebral disc degeneration
Target | Forward primer | Probe | Reverse primer | Threshold |
|---|---|---|---|---|
GAPDH | PDAR | PDAR | PDAR | 0.047 |
Collagen type I | 5' CAG CCG CTT CAC CTA CAG C 3' | 5' CCG GTG TGA CTC GTG CAG CCA TC 3' | 5' TTT TGT ATT CAA TCA CTG TCT TGC C 3' | 0.078 |
Collagen type II | 5' GGC AAT AGC AGG TTC ACG TAC A 3' | 5' CCG GTA TGT TTC GTG CAG CCA TCC T 3' | 5' CGA TAA CAG TCT TGC CCC ACT T 3' | 0.100 |
Aggrecan | 5' TCG AGG ACA GCG AGG CC 3' | 5' ATG GAA CAC GAT GCC TTT CAC CAC GA 3' | 5' TCG AGG GTG TAG CGT GTA GAG A 3' | 0.050 |
SOX9 | 5' GAC TTC CGC GAC GTG GAC 3' | 5' CGA CGT CAT CTC CAA CAT CGA GAC 3' | 5' GTT GGG CGG CAG GTA CTG 3' | 0.0562 |
SOX6 | 5' CCG TGA GAT AAT GAC CAG TGT TAC TT 3' | 5' AAC CCC AGA GCG CCG CAA A 3' | 5' GTC CAC CAC ATC GGC AAG AC 3' | 0.052 |
IL-1α | PDAR | PDAR | PDAR | 0.107 |
IL-1β | PDAR | PDAR | PDAR | 0.122 |
IL-1Ra | 5' CCT GCA GGG CCA AGC A 3' | 5' AGC CTC GCT CTT GGC AGG TAC TCA GT 3' | 5' GCA CCC AAC ATA TAC AGC ATT CA 3' | 0.122 |
IL-1RI | 5' ATT TCT GGC TTC TAG TCT GGT GTT C 3' | 5' ACT TGA TTT CAG GTC AAT AAC GGT CCC C 3' | 5' AAC GTG CCA GTG TGG AGT GA 3' | 0.163 |
MMP-3 | 5' TGA AGA GTC TTC CAA TCC TAC TGT TG 3' | 5' CGT GGC AGT TTG CTC AGC CTA TCC AT 3' | 5' CTA GAT ATT TCT GAA CAA GGT TCA TGC A 3' | 0.108 |
MMP-9 | 5' CCC GGA GTG AGT TGA ACC A 3' | 5' CCA AGT GGG CTA CGT GAC CTA TGA CAT CC 3' | 5' CAG GAC GGG AGC CCT AGT C 3' | 0.041 |
MMP-13 | 5' GGA CAA GTA GTT CCA AAG GCT ACA A 3' | 5' CTC CAA GGA CCC TGG AGC ACT CAT GTT 3' | 5' CTT TTG CCG GTG TAG GTG TAG ATA G 3' | 0.108 |
ADAMTS-4 | 5' ACT GGT GGT GGC AGA TGA CA 3' | 5' ATG GCC GCA TTC CAC GGT G 3' | 5' TCA CTG TTA GCA GGT AGC GCT TT 3' | 0.052 |
ADAMTS-5 | 5' GGA CCT ACC ACG AAA GCA GAT C 3' | 5' CCC AGG ACA GAC CTA CGA TGC CAC C 3' | 5' GCC GGG ACA CAC GGA GTA 3' | 0.122 |