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Table 1 Summary of results from two separate experiments implanting CHO cells into SCID mice

From: Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity

Implanted cells Mean human IgG in sera (ng/ml) (no. of mice/group) Terminal proteinuria (range for group) Mean proteinuria (estimateda g/l) Mean day of death
Experiment 1     
   CHO-B3 <16 (3/5), 0 (2/5) ++/+++ to +++ 2.4 20
   CHO-B3(R27aS) 260 (2/5), <16 (3/5) + to ++ 0.65 24
   Nontransfected CHO only 0 Trace to trace/+ 0.2 34b,c
   Pristane only 0 Trace/+ 0.2 34b
Experiment 2     
   CHO-B3 105 (4/5), NR (1/5) ++ to +++ 2.4 26
   CHO-B3(R27aS) 420 (5/5) + to ++/+++ 0.99 39
   CHO with empty vector 0 Trace to +/++ 0.38 48
   Pristane only 0 Trace/+ 0.2 56b
  1. aProteinuria was assessed using Albustix, which give a semiquantitative measure based on colour change. Based on values provided with the Albustix, in order to calculate the estimated proteinuria in g/l we assumed that trace = 0.1 g/l, trace/+ = 0.2 g/l, +/++ = 0.65 g/l, and ++/+++ = 2.0 g/l. The proteinuria data in both experiments were found to be normally distributed using the Kolmogorov–Smirnov test and compared using a one-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison test. In experiment 1, the ANOVA showed that there was a significant difference between the mean proteinuria values of all the groups (P < 0.0001). The Bonferroni post test indicated that there was a significant difference between mice implanted with CHO-B3 and CHO-B3(R27aS) (P < 0.001), nontransfected CHOdhfr- cells (P < 0.001), and pristane only (P < 0.001). In experiment 2, the ANOVA showed that there was a significant difference between the mean proteinuria values of all the groups (P < 0.0003). The Bonferroni post test indicated that there was a significant difference between mice implanted with CHO-B3 and CHO-B3(R27aS) (P < 0.05), CHO cells containing the empty vector (P < 0.001), and pristane only (P < 0.01). bMice still healthy at the end of the experiment. cThese cells did not survive/grow in the mice. CHO, Chinese hamster ovary; NR, not recorded; SCID, severe combined immunodeficiency.