Conversion of ASD and testosterone into downstream steroid hormones in mixed synovial cells. (a,b) Spontaneous conversion of radiolabeled ASD into downstream metabolites in OA (open bars, n = 23) and RA patients (hatched bars, n = 19). (c,d) Spontaneous conversion of radiolabeled testosterone into downstream metabolites in OA (open bars, n = 10) and RA patients (hatched bars, n = 9). All panels: the E3 and 16αOH-testerone produced are given in one bar because only one spot was detected for both steroids using TLC. Values are given as pmol/106 cells/48 hr as box blots with the 5th, 25th, 50th (median), 75th, and 95th percentile when applicable. * Denotes radiolabeled substrate. OA, osteoarthritis; RA, rheumatoid arthritis. Other abbreviations are as given in the legend to Fig. 1.