Protective effect of vasoactive intestinal peptide on bone destruction in the collagen-induced arthritis model of rheumatoid arthritis

Table 1 Primer sequences for several factors involved in bone regulation and for β-actin

Gene name	Genebank accession number	Sequence position	Primers	Sequence
β-Actin	NM007393	694–831	Bactin.for Bactin.rev	5'-AGAGGGAAATCGTGCGTGAC-3' 5'-CAATAGTGATGACCTGGCCGT-3'
IL-11	NM008350	350–450	IL-11.for IL-11.rev	5'-TGATGTCCTACCTCCGGCAT-3' 5'-TTCCAGTCGGGCTTGCAG-3'
IL-17	NM010552	146–246	IL-17.for IL-17.rev	5'-CCTCAAAGCTCAGCGTGTCC-3' 5'-GAGCTCACTTTTGCGCCAAG-3'
COX-2	NM011198	854–954	COX-2.for COX-2.rev	5'-GGTGGAGAGGTGTATCCCCC-3' 5'-ACTTCCTGCCCCACAGCA-3'
iNOS	NM010927	872–972	iNOS.for iNOS.rev	5'-AACAATGGCAACATCAGGTCG-3' 5'-CCAGCGTACCGGATGAGCT-3'
OPG	U94331	831–931	OPG.for OPG.rev	5'-AGAGCAAACCTTCCAGCTGC-3' 5'-CGCTGCTTTCACAGAGGTCA-3'
RANK	AF19046	1422–1440	RANK.for RANK.rev	5'-TGCCTACAGCATGGGCTTT-3' 5'AGAGATGAACGTGGAGTTACTGTTT3'
RANKL	AF53713	606–680	RANKL.for RANKL.rev	5'-TGGAAGGCTCATGGTTGGAT-3' 5'-CATTGATGGTGAGGTGTGCAA-3'

COX-2, cyclooxygenase-2; iNOS, inducible nitric oxide synthase; OPG, osteoprotegerin; RANK, receptor activator of nuclear factor-κB; RANKL, receptor activator of nuclear factor-κB ligand.

ISSN: 1478-6362