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Table 1 Sequences of the primers used for RT-PCR

From: Expression of ADAM15 in rheumatoid synovium: up-regulation by vascular endothelial growth factor and possible implications for angiogenesis

Primer's name

Product

Oligonucleotide sequence

Size (base pairs)

Accession number

ADAM8

Forward

5'-GCCGTCTTCAGGCCTCGGCCCGGGGACTCT-3'

651

NM001109

 

Reverse

5'-AGGGGCGTTGGCGAGGCACACCGACTGCGG-3'

  

ADAM9

Forward

5'-GCTGTCTTGCCACAGACCCGGTATGTGGAG-3'

604

HSU41766

 

Reverse

5'-TGGAATATTAAGAAGGCAGTTTCCTCCTTT-3'

  

ADAM10

Forward

5'-ATCCAGTCATGTTAAAGCGATTGATACAATTTAC-3'

434

NM001110

 

Reverse

5'-TCCAAAGTTATGTCCAACTTCGTGAGCAAAAGTAA-3'

  

ADAM12

Forward

5'-GAGACCCTCAAGGCAACTAAGTATGTGGAG-3'

627

AF023476

 

Reverse

5'-CGGCAGGTTAAACAGGCACACCCCCATTCC-3'

  

ADAM15

Forward

5'-CTGGGACAGCGCCACATTCGCCGGAGGCGG-3'

688

HSU41767

 

Reverse

5'-TCCGCAGAAAGCAGCCATAGGGGGTAGGCT-3'

  

ADAM17

Forward

5'-AGAGCTGACCCAGATCCCATGAAGAACACG-3'

777

HSU69611

 

Reverse

5'-GCGTTCTTGAAAACACTCCTGGGCCTTACT-3'

  

ADAM20

Forward

5'-AAAATAGCACACCAGATGGAGTTGCAATTG-3'

702

AF029899

 

Reverse

5'-ATTCCCACAGTACTTCAGTCTAAATATATT-3'

  

ADAM21

Forward

5'-TCTGGCTTGGGGTATTTTTG-3'

500

AF158644

 

Reverse

5'-TTGGCGTGCTACTTCCTTCT-3'

  

ADAM28

Forward

5'-GCTGTGATGCTAAGACATGT-3'

871

AF137334

 

Reverse

5'-TGAACAGCCTTTACCATCTG-3'

  

ADAM30

Forward

5'-AACCAGGTGCCAACTGTAGC-3'

496

AF171932

 

Reverse

5'-CCCATGGGTTTCATGGATAG-3'

  

VEGFR-1

Forward

5'-GATGTTGAGGAAGAGGAGGATT-3'

1146

NM002019

 

Reverse

5'-AAGCTAGTTTCCTGGGGGTATA-3'

  

VEGFR-2

Forward

5'-GATGTGGTTCTGAGTCCGTCT-3'

562

NM002253

 

Reverse

5'-CATGGCTCTGCTTCTCCTTTG-3'

  

Neuropilin-1

Forward

5'-CAACGATAAATGTGGCGATACT-3'

824

NM003873

 

Reverse

5'-TATACTGGGAAGAAGCTGTGAT-3'

  

CD31

Forward

5'-CAACGAGAAAATGTCAGA-3'

259

NM000442

 

Reverse

5'-GGAGCCTTCCGTTCTAGAGT-3'

  

vWF

Forward

5'-GTTCGTCCTGGAAGGATCGG-3'

697

NM000552

 

Reverse

5'-CACTGACACCTGAGTGAGAC-3'

  

β-Actin

Forward

5'-TGACGGGGTCACCCACACTGTGCCCATCTA-3'

661

NM001101

 

Reverse

5'-CTAGAAGCATTTGCGGTGGACGATGGAGGG-3'

  
  1. ADAM, a disintegrin and metalloproteinase; VEGFR, vascular endothelial growth factor receptor; vWF, von Willebrand factor.
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Arthritis Research & Therapy

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