Figure 5
From: Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis
IL-6 levels in serum and in CXCL12-stimulated splenocyte cultures. (a) Eight mice were immunized with collagen type II/complete Freund's adjuvant (CIA) and implanted with osmotic minipumps delivering AMD3100 (four mice) at a constant rate of 600 μg/day or containing PBS (four mice). Blood was collected at day 10 of the treatment. Sera were pooled in each group and analysed for the presence of IL-6 (using a SearchLight Proteome array). Bars represent the average ± standard error of the mean of two independent experiments. (b) Splenocytes of three collagen type II/complete Freund's adjuvant-immunized mice were pooled and cultured in the absence of CXCL12, in the presence of CXCL12 (0.5 μg/ml) or in the presence of CXCL12 and AMD3100 (25 μg/ml). Supernatant was analysed after 48 h for the presence of IL-6. Bars represent the average ± standard error of the mean of three independent experiments.