Figure 3From: Raloxifene reduces urokinase-type plasminogen activator-dependent proliferation of synoviocytes from patients with rheumatoid arthritisFibrinolytic pattern of healthy (H) and rheumatoid arthritis (RA) synoviocytes treated with Raloxifene. Samples were analyzed both in basal conditions and after treatment with 0.5 and 1 μM Raloxifene (48 h). (a) Urokinase-type plasminogen activator (u-PA) antigen was quantified on aliquots of the culture medium by ELISA assay and reported as ng/106 synovial cells. (b) u-PA receptor (u-PAR) was quantified by ELISA assay on aliquots of cell lysates and reported as ng/106 synovial cells. (c) Plasminogen activator inhibitor (PAI)-1 was also measured in aliquots of the culture medium, and reported as μg/106 synovial cells. H refers to four different synovial cell cultures from healthy individuals, RA refers to synovial cell cultures from four different rheumatoid arthritis patients. In (a-c), each point represents the mean ± standard deviation of three experiments performed in triplicate on each synovial cell line. § = p < 0.01 versus basal (0.2% FCS).Back to article page