Fibrinolytic pattern of healthy (H) and rheumatoid arthritis (RA) synoviocytes treated with Raloxifene. Samples were analyzed both in basal conditions and after treatment with 0.5 and 1 μM Raloxifene (48 h). (a) Urokinase-type plasminogen activator (u-PA) antigen was quantified on aliquots of the culture medium by ELISA assay and reported as ng/106 synovial cells. (b) u-PA receptor (u-PAR) was quantified by ELISA assay on aliquots of cell lysates and reported as ng/106 synovial cells. (c) Plasminogen activator inhibitor (PAI)-1 was also measured in aliquots of the culture medium, and reported as μg/106 synovial cells. H refers to four different synovial cell cultures from healthy individuals, RA refers to synovial cell cultures from four different rheumatoid arthritis patients. In (a-c), each point represents the mean ± standard deviation of three experiments performed in triplicate on each synovial cell line. § = p < 0.01 versus basal (0.2% FCS).