Lysozyme and its biological value in rheumatoid arthritis (RA)
© BioMed Central Ltd 2001
Received: 15 January 2001
Published: 26 January 2001
Lysozyme or muramidase catalyzes the hydrolysis of 1,4-beta-linkages between N-aacetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan. A basic enzyme that is present in saliva, tears, egg white and many animal fluids. Its function is an antibacterial agent. Lysozyme is well known for the ability to hydrolize the cell wall of bacteria.
The aim of study was to measure the concentration of lysozyme in synovial fluid in RA patients.
We measured the lytic activity of lysozyme towards micrococcus lysodeikticus (1, 2, 3), bacteria which are highly suspectible to lysis by lysozyme by the turbidometric method 30 synovial fluid of RA patients. In order to obtain a method covering a wider range of lysozyme concentrations, Osserman and Lawlor worked out the so-called lyso-plate method (4).
The test measured the zone of clearing by lysozyme in an agar plate, in which microccocus lysodeikticus is embedded. After about 18 hours the diameter of the zone of clearing is measured.
In all our RA synovial fluid we observed increased level of lysozyme.
The increased levels of lysozyme in synovial fluid in RA could indicate of monocyte/macrofage activity and might be used to study disease activity in RA.
- Smolelis AN, Hartsell SE: The determination of lysozyme. J Bacteriol.,. 1949, 731: 58-Google Scholar
- Litwack G: Photometric determination of lysozyme activity. Proc. Soc. Exp. Biol. (NY),. 1955, 89: 401-View ArticleGoogle Scholar
- Prockop DJ, Davidson WD: A study of urinary and serum lysozyme in patients with renal disease. New Engl J Med,. 1964, 270: 269-View ArticleGoogle Scholar
- Osserman EF, Lawlor DP: Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med. 1966, 124: 921-10.1084/jem.124.5.921.View ArticlePubMedPubMed CentralGoogle Scholar
- Torsteinsdottir I, Hakansson L, Hallgren R, et al: Serum lysozyme: a potential marker of monocyte/macrophage activity in rheumatoid arthritis. Rheumatology - Oxford,. 1999, 38: 1949-View ArticleGoogle Scholar