Suppression of ICAM-1 expression by DHMEQ. Shown, using flow cytometry, is suppression of intercellular adhesion molecule (ICAM)-1 expressed on tumor necrosis factor (TNF)-α-stimulated rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) by dehydroxymethylepoxyquinomicin (DHMEQ). Cells were preincubated for 20 minutes with 10 μg/ml DHMEQ or vehicle. TNF-α stimulated or unstimulated RA FLS were incubated with isotype-matched control IgG or anti-ICAM-1 antibody, followed by phycoerythrin-labeled second antibody. (a) Representative data are shown, along with (b) the means ± standard deviation of ICAM-1-positive cells in four independent experiments.