Figure 3

Surface expression of CD14, TLR4 and TLR2 on CD4⁺CD28⁺ and CD28^null cells in ankylosing spondylitis, psoriatic arthritis and rheumatoid arthritis. (a) Representative dot plots and histograms show TLR4 expression (filled red curve, black line represents isotype control) on CD4⁺CD28⁺ and CD4⁺CD28^null T cells. Gates were set on lymphocytes (forward scatter and sideward scatter) as well as on CD28⁺ and CD28^null cells expressing high levels of CD4. (b) Box plots summarize the expression of CD14, TLR4 and TLR2 on CD4⁺CD28⁺ and CD28^null T cells in patients as indicated. The Wilcoxon test was used to determine the statistical differences between the groups. ***P < 0.001. SSC, side scatter; FSC, forward scatter; AS, ankylosing spondylitis; PsA, psoriatic arthritis; RA, rheumatoid arthritis.