TaqMan validation of the expression of the selected biomarker candidates. TaqMan RT-PCR was performed using primer and probe sets specific to (a) Galectin-3, (b) Veriscan/Cspg2, and (c) Socs3. (d) IL-6, an acute responding gene that has not been selected from the microarray analysis, was also assayed as a control. The RNA samples are independent from the ones used for microarray analysis, and more time points were used in the PCR analysis. Data are expressed as mean ± standard error. The number of the samples assayed for each group is marked in the parenthesis above the histogram. * P < 0.05, by Dunnet's test. CII, collagen type II.