Figure 3

AddnIκB-PEG-RGD infected human umbilical vein endothelial cells (HUVECs) express dnIκB in a knob-independent, RGD-dependent manner. HUVECs were incubated with either non-modified AddnIκB, AddnIκB-PEG-RGD or AddnIκB-PEG-RAD (3,000 vp/cell) for 90 minutes, in the absence or presence of either 20 mg/ml recombinant viral knob or 50 mg/ml RGD-protein or both, as described in Materials and methods. Cells were subsequently washed and incubated for another 24 h. After preparation of cellular protein homogenate, western blotting was performed to detect the hemagglutinin-tagged transgene, and actin to control for protein loading.