Figure 6

Regulation of CD40 expression is not intrinsically altered in lupus DCs. We grew bone marrow-derived dendritic cells (BM-DCs) from NZM2410, BALB/c, and B6 mice 6 to 9 months old and stained them at day 6 to 7 of culture. Gating of CD11c⁺ cells (a) and CD40 expression (b) in resting BM-DCs from NZM2410 (thick dark grey line), BALB/c (light grey area), and B6 (thin black line) mice. (c) Percentages of CD40-positive BM-DCs, gated as in (a) and considered CD40-positive using the threshold shown in (b) from three experiments, conducted with three independent BM-DCs cultures. Error bars show SD. (d,e) Mean fluorescence intensity (MFI) of CD40 expression in splenic myeloid DCs isolated and cultured overnight in vitro from NZM2410 (d) and NZB-W/F1 (e) mice 6 to 9 months old.