| |
Wild-type mice (pg/ml ± SEM)
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A2A knockout mice (pg/ml ± SEM)
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A3 knockout mice (pg/ml ± SEM)
|
|---|
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Control
|
42 ± 7 (n = 14)
|
36 ± 8 (n = 10)
|
75 ± 18* (n = 6)
|
|
Methotrexate (0.75 mg/kg/week)
|
14 ± 4** (n = 15)
|
25 ± 7 (n = 9)
|
31 ± 11† (n = 8)
|
- Wild-type mice, A2A receptor knockout mice or A3 receptor knockout mice were treated with either weekly injections of methotrexate (0.75 mg/kg) or saline control for 4 weeks prior to induction of inflammation. Inflammatory exudates were induced in the peritoneum of mice, as described. After 4 hours the exudates were collected, centrifuged at 100 × g and frozen. Tumor necrosis factor alpha levels were later quantitated by ELISA. Wild-type data are a combination from both mouse strains. **P < 0.001 vs wild-type control mice, Student's t test; *P < 0.05 vs wild-type control mice, Student's t test; †P < 0.05 vs A3 knockout control mice, Student's t test.
