Figure 3
Comparison of the expression of nuclear factor (NF)κB1 (p50) protein with that of NFκB1 mRNA in bone marrow CD34+ cells. Purified bone marrow CD34+ cells were permeabilized and then stained with phychoerythrin-conjugated anti-NFκB p50 monoclonal antibody or phychoerythrin-conjugated normal mouse IgG1, followed by analysis with flow cytometry. The NFκB1 protein levels as expressed by mean fluorescence intensity were compared with NFκB1 mRNA levels (expressed as the ratio of the mRNA copy numbers to those of β-actin) in bone marrow CD34+ cells from six patients (three rheumatoid arthritis patients and three osteoarthritis patients). Statistical significance was evaluated by linear regression test.