The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes

Table 2 IL-1F8 protein levels in mouse and human joint samples and in mouse skin

Samples	Patients/animals	IL-1F8 (pg/mg protein)	IL-1β (pg/mg protein)
Human synovium	OA (n = 4)	3.7 ± 1.4	3.6 ± 0.3
	Inflammatory arthritis (n = 5)	3.9 ± 1.0	11.6 ± 5.7^a
Mouse joint	Naïve (n = 1)	2.8	2.3
	Nonarthritic (n = 1)	2.7	4.5
	CIA early^b (n = 3)	2.2 ± 0.3	16.6 ± 1.2^c
	CIA late^b (n = 3)	4.5 ± 1.4	8.8 ± 0.8
Mouse skin	Control (n = 3)	761.7 ± 313.4	16 ± 3
	PMA (n = 3)	14569.1 ± 3632.5^d	28 ± 3^d

IL-1F8 protein levels were determined using enzyme-linked immunosorbent assay. ^aP < 0.05 versus osteoparthritis (OA), as assessed using the Mann-Whitney test. ^bCollagen-induced arthritis (CIA) early: days 1–7 after the onset of arthritis; CIA late: days 8–21 after onset of arthritis. ^cP < 0.05 versus late CIA, as assessed using analysis of variance. ^dP < 0.05 versus control, as assessed using analysis of variance.

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