Figure 5
From: Inhibition of protein geranylgeranylation induces apoptosis in synovial fibroblasts
Geranylgeranylated proteins are redistributed to the cytoplasm in the presence of statin. Synovial fibroblasts were cultured for 72 hours in the presence of 3 μM simvastatin supplemented or not with 5 μM farnesylpyrophosphate (FPP) or 5 μM geranylgeranylpyrophosphate (GGPP). Phase separation of proteins was achieved by partitioning with Triton X-114. The entire membrane and cytoplasmic fractions were subjected to PAGE on a 12% gel. Blots were probed with mouse anti-human RhoA or Rac1 followed by goat anti-mouse IgG conjugated to horseradish peroxidase secondary antibody, and were revealed by enhanced chemiluminescence.