Figure 4From: Modulation of granulocyte-endothelium interactions by antileukoproteinase: inhibition of anti-type II collagen antibody-induced leukocyte attachment to the synovial endotheliumInhibition of leukocyte attachment to IL-1β-treated EA.hy 926 cells by antileukoproteinase in vitro. Adherence of human CFDA-SE (carboxyfluorescein diacetate succinimidyl diester)-labelled granulocytes to layers of EA.hy 926 cells was assessed by fluorescence microscopy. A comparison of granulocyte adherence to EA.hy 926 cells that were either untreated (- IL-1β) or cytokine-stimulated (+IL-1β) is shown. Pretreatment of EA.hy 926 cells with 5 μM antileukoproteinase (ALP) (+IL-1β +ALP) but not with 5 μM hSA (+IL-1β +hSA) resulted in a marked decrease in granulocyte adhesion.Back to article page