Figure 3

Osteoclast differentiation induced in vitro by macrophage colony-stimulating factor and RANKL. Cells were transiently transferred with ribbon-type scrambled decoy oligodeoxynucleotide (RSODN) (b) or ribbon-type NF-κB decoy oligodeoxynucleotide (RNODN) (c), or were untreated alone (a). Original magnification × 100. (d) Numbers of TRAP-positive multinuclear cells. (n = 5 per group; *p < 0.001, compared with rats treated with RSODN.) (e-h) Calcified matrix resorption by osteoclast-like cells induced by soluble receptor activator of nuclear factor κB ligand (RANKL). Cells were transiently transferred with RSODN (f) or RNODN (g) or were untreated alone (e). (h) Mean calcified matrix resorption areas calculated by MacSCOPE image analyzer. (n = 5 per group; *p < 0.01 compared with the RSODN-treated group.)