Figure 6

Histological analysis in the ankle joints of rats with collagen-induced arthritis (CIA) at day 35. Samples were stained with hematoxylin and eosin in (a-d) and with tartrate-resistant acid phosphatase [TRAP] in (e-h). (a) Naive rats had normal joints. (b) Ankle joints of rats with CIA treated with PBS showed pannus invasion and massive cellular infiltration of the synovium, with disruption of cartilage and subchondral bone. (c) Ankle joints of rats with CIA treated with ribbon-type scrambled decoy oligodeoxynucleotide (RSODN) also showed pannus invasion and massive cellular infiltration of the synovium, with disruption of cartilage and subchondral bone. (d) Ankle joints of rats with CIA treated with ribbon-type NF-κB decoy oligodeoxynucleotide (RNODN) also had nearly intact articular joints. (e) Ankle joints of naive rats had few TRAP-positive multinuclear cells. (f) Ankle joints of rats with CIA treated with PBS showed active resorption of cartilage and subchondral bone by pannus and synovium including TRAP-positive multinuclear cells. (g) Ankle joints of rats with CIA treated with RSODN also showed active resorption of cartilage and subchondral bone by pannus and synovium including TRAP-positive multinuclear cells. (h) TRAP-positive multinuclear cell formation was suppressed in ankle joints of rats with CIA treated with RNODN. Original magnifications × 100.