Figure 3
From: Characterisation of the immune response to type I collagen in scleroderma
Th1 polarisation of type I collagen (CI)-responsive T-cell lines. Cytokine expression of T-cell lines was determined by intracellular (IC) staining of PMA (phorbol 12-myristate 13-acetate)/ionomycin activated T cells. (a) We detected interferon (IFN)-γ staining but no interleukin (IL)-4 staining. Multiplex cytokine assay was used to analyse the cytokine profile of three T-cell line supernatants. (b) An excess of Th1 over Th2 cytokines was detected. PE, phycoerythrin; TNF, tumour necrosis factor.