Figure 2

Validation of microarray data with quantitative real-time polymerase chain reaction (QRT-PCR) showing a representative experiment (repeated one additional time). Three controls and three patients were selected for QRT-PCR to validate microarray results. QRT-PCR was carried out for individual samples, and then the average threshold cycle (Ct) of the patients and the average Ct of the healthy controls were used to calculate relative expression, expressed as fold change. The fold changes of both microarray (open bars) and QRT-PCR (solid bars) are shown. For all six genes selected, relative expression was higher in healthy controls relative to patients as shown by microarrays and QRT-PCR, thus confirming the microarray results.