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Table 2 The dynamic range, standard curve slope, R2 value, and efficiency of each polymerase chain reaction assay

From: Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction

Assay Lower detection limit (CT value) Upper detection limit (CT value) Standard curve slope R2 value Efficiency
ADAMTS5 26.0 35.9 -3.32 0.99 100.2
AGC 18.5 34.7 -3.29 0.99 101.5
BGN 20.8 34.8 -3.49 1.00 93.3
COL1A2 17.4 33.5 -3.30 1.00 101.0
COL2A1 22.7 32.2 -3.22 1.00 104.6
COL3A1 16.5 33.0 -3.33 1.00 99.9
COL5A1 23.2 33.1 -3.31 1.00 100.5
COL9A3 26.3 32.7 -3.22 1.00 104.8
CSPG2 21.4 34.3 -3.25 1.00 103.2
CTSB 19.7 32.6 -3.24 1.00 103.3
CTSD 24.1 34.2 -3.29 1.00 101.5
DCN 19.0 31.9 -3.25 1.00 103.0
GAPDH 22.7 35.2 -3.27 0.99 102.3
Genomic 16.8 40.0 -4.42 1.00 68.3
LUM 19.9 33.7 -3.48 1.00 93.9
MMP13 26.1 36.3 -3.36 0.98 98.6
RPL13A 18.6 32.1 -3.36 1.00 98.6
SDHA 21.6 34.6 -3.26 1.00 102.5
TBP 16.5 30.0 -3.39 1.00 97.4
TIMP1 22.6 33.1 -3.48 1.00 93.7
TIMP2 21.8 32.1 -3.43 1.00 95.7
TIMP4 29.5 35.8 -3.16 0.99 107.4
TNC 20.1 33.0 -3.26 1.00 102.5
VIM 15.8 32.7 -3.35 1.00 98.8
  1. See Additional file 1 for further details. ADAMTS5, ADAM metallopeptidase with thrombospondin type 1 motif, 5; AGC1, aggrecan; BGN, biglycan; COL1A2, type I collagen, alpha two chain; COL2A1, type II collagen alpha 1 chain; COL3A1, type III collagen alpha 1 chain; COL5A1, type V collagen alpha 1 chain; COL9A3, type IX collagen alpha 3 chain; CSPG2, chondroitin sulphate proteoglycan 2; CTSB, cathepsin B; CTSD, cathepsin D; DCN, decorin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LUM, lumican;MMP13, matrix metalloproteinase 13; RPL13A, ribosomal protein L13a; SDHA, succinate dehydrogenase complex, subunit A; TBP, TATA box binding protein; TIMP1, tissue inhibitor of metalloproteinase 1; TIMP2, tissue inhibitor of metalloproteinase 2; TIMP4, tissue inhibitor of metalloproteinase 4; TNC, Tenascin C; VIM, vimentin.