Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction

Table 2 The dynamic range, standard curve slope, R² value, and efficiency of each polymerase chain reaction assay

Assay	Lower detection limit (C_T value)	Upper detection limit (C_T value)	Standard curve slope	R² value	Efficiency
ADAMTS5	26.0	35.9	-3.32	0.99	100.2
AGC	18.5	34.7	-3.29	0.99	101.5
BGN	20.8	34.8	-3.49	1.00	93.3
COL1A2	17.4	33.5	-3.30	1.00	101.0
COL2A1	22.7	32.2	-3.22	1.00	104.6
COL3A1	16.5	33.0	-3.33	1.00	99.9
COL5A1	23.2	33.1	-3.31	1.00	100.5
COL9A3	26.3	32.7	-3.22	1.00	104.8
CSPG2	21.4	34.3	-3.25	1.00	103.2
CTSB	19.7	32.6	-3.24	1.00	103.3
CTSD	24.1	34.2	-3.29	1.00	101.5
DCN	19.0	31.9	-3.25	1.00	103.0
GAPDH	22.7	35.2	-3.27	0.99	102.3
Genomic	16.8	40.0	-4.42	1.00	68.3
LUM	19.9	33.7	-3.48	1.00	93.9
MMP13	26.1	36.3	-3.36	0.98	98.6
RPL13A	18.6	32.1	-3.36	1.00	98.6
SDHA	21.6	34.6	-3.26	1.00	102.5
TBP	16.5	30.0	-3.39	1.00	97.4
TIMP1	22.6	33.1	-3.48	1.00	93.7
TIMP2	21.8	32.1	-3.43	1.00	95.7
TIMP4	29.5	35.8	-3.16	0.99	107.4
TNC	20.1	33.0	-3.26	1.00	102.5
VIM	15.8	32.7	-3.35	1.00	98.8

See Additional file 1 for further details. ADAMTS5, ADAM metallopeptidase with thrombospondin type 1 motif, 5; AGC1, aggrecan; BGN, biglycan; COL1A2, type I collagen, alpha two chain; COL2A1, type II collagen alpha 1 chain; COL3A1, type III collagen alpha 1 chain; COL5A1, type V collagen alpha 1 chain; COL9A3, type IX collagen alpha 3 chain; CSPG2, chondroitin sulphate proteoglycan 2; CTSB, cathepsin B; CTSD, cathepsin D; DCN, decorin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LUM, lumican;MMP13, matrix metalloproteinase 13; RPL13A, ribosomal protein L13a; SDHA, succinate dehydrogenase complex, subunit A; TBP, TATA box binding protein; TIMP1, tissue inhibitor of metalloproteinase 1; TIMP2, tissue inhibitor of metalloproteinase 2; TIMP4, tissue inhibitor of metalloproteinase 4; TNC, Tenascin C; VIM, vimentin.

ISSN: 1478-6362