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Table 4 BALF proteins whose regulation in SScFib+ and SScFib- patients is different

From: Analysis of bronchoalveolar lavage fluid proteome from systemic sclerosis patients with or without functional, clinical and radiological signs of lung fibrosis

Spot no. Median (IQR) spot quantity Protein or fragment MW (kDa) pI AC Identification method
  SScFib+ SScFib- p      
13 328.0 (256.5–482.5) 86.0 (68.6–110.4) 0.0003 α1-acid glycoprotein (orosomucoid) 23.5 4.93 P02763 GM (plasma, BALF), Ab
19 7.9 (5.6–9.6) 28.2 (24.5–32.6)   Cu–Zn superoxide dismutase 15.8 5.70 P00441 GM (plasma, BALF), Ab
35 343.0 (328.5–664.0) 168.0 (131.5–267.0) 0.0041 Serum albumina 69.4 5.92 P02768 MS
36 96.6 (68.9–127.6) 24.4 (17.2–64.5) 0.0012 Calgranulin B (S100A9; MRP14) 13.2 5.71 P06702 MS, GM (plasma, BALF)
37 53.3 (47.4–72.1) 11.1 (8.0–22.9) 0.0001 Haptoglobin (Hp2) α chaina 15.9 5.57 P00738 MS, LC, GM (plasma, BALF)
38 Not expressed 32.0 (29.5–39.0)   Glutathione S transferase P 23.2 5.44 P09211 MS, GM (plasma, BALF)
39 59.1 (50.8–73.1) 14.8 (9.4–19.6) 0.0014 Cytohesin-2a,b 46.5 5.43 Q99418 MS, LC MS, LC
     Calumenina,b 37.1 4.47 O43852 MS, LC MS, LC
40 29.2 (25.5–30.8) Not expressed   Mitochondrial DNA topoisomerase 1a,b 69.8 9.46 Q969P6 MS, LC
41 Not expressed 45.5 (32.2–58.9)   Cystatin SNb 16.3 6.82 P01037 MS, LC
42 Not expressed 37.7 (34.0–41.0)   Not determined - - - (MS, LC)
  1. Spot quantity values were measured on two BALF gel maps of individual SScFib+ (n = 9) and SScFib- (n = 6) patients by PDQUEST 7.1 software. Results are expressed as median and interquartile range (IQR). Spot no. refers to the annotations in Figure 1; p values were calculated with the Mann–Whitney U test. BALF, bronchoalveolar lavage fluid; SScFib+, systemic sclerosis patients with functional lung fibrosis and signs of lung fibrosis on high-resolution computed tomography; SScFib-, SSc patients with no signs and symptoms of lung fibrosis; MW, theoretical average molecular mass; pI, isoelectric point; AC, accession number from the Swiss-PROT 2DPAGE database [17]; GM, gel matching with plasma two-dimensional gel electrophoresis database from Swiss-PROT [17] and published BALF two-dimensional gel electrophoresis maps [5,6]; Ab, immunoblotting; MS, matrix-assisted laser desorption/ionization mass spectrometry analysis; LC, liquid chromatography–tandem mass spectrometry analysis. aFragments. bProteins not previously described in published BALF two-dimensional gel electrophoresis maps.