The expression of Synoviolin (SYN) during collagen-induced arthritis (CIA) development. (a) Synovial fibroblasts were isolated from normal DBA mice (Normal) and DBA mice 20 days after the first collagen immunization (Immunized) or 10 days after the second immunization when CIA is developed (CIA). The expression of SYN in those synovial fibroblasts was analyzed by Western blotting. (b) The densities of each band in (a) were analyzed, and the ratio of SYN/(10-1 actin) was used. Error bars represent three different CIA, immunized, or normal mice. Statistic analysis indicates that the expression of SYN is significantly increased during CIA development.