Table 5 Genes whose expression showed the greatest change following incubation of chondrocytes with elevated IL-1β alone: genes of known function whose expression was most affected by IL-1 alone

• A disintegrin and metaloproteinase domain 33

• c-fos-induced growth factor (vascular endothelial growth factor D)

Kruppel associated box zinc finger 1

• Smoothelin

• Actin alpha 1

• Collagen type II alpha 1, chain precursor

• Microfibril-associated glycoprotein precursor

Solute carrier family 39 (iron-regulated transporter), member 1

Aggrecan 1

Crystallin, alpha B

• Midline 1

• Sphingomyelin phosphodiesterase 3, neutral

• Ankyrin-like repeat protein

Distal-less homeobox

• Mitochondrial ribosomal protein L53

• Transforming growth factor, beta 2

• Annexin III (Lipocortin III)

• DVS27-related protein

Myocilin precursor

• Zinc finger protein SLUG (neutral crest transcription factor Slug)

Cadherin-8 precursor

• Dynein, cytoplasmic, intermediate chain 1

• NAD-dependent 15-hydroxyprostaglandin dehydrogenase

Calpain 6

• Enolase 3, beta

Osteomodulin (osteoadherin)

• Carbonic anhydrase 3

Four and a half LIM domains 1

• Palmdelphin^a

Cartilage link protein 1 ^a

• Heat shock protein HSP 90 beta^a

• Phosphoribosyl pyrophosphate synthestase 2

• Caveolin 3

Insulin-like growth factor binding protein 4 precursor

• Programmed cell death protein 7

Genes whose RNA levels were increased more than fivefold relative to untreated controls

Adaptor protein with pleckstrin homology and src homology 2 domains

Cyclooxygenase-2

Keratin, type II cryosqueletal 8

Plasminogen activator, urokinase receptor

Adenosine A2B receptor

Cytochrome P450, family 26, subfamily b, polypeptide 1

Lactose operon repressor

Prostaglandin E synthase ^a

Adenosine monophosphate deaminase 3

Cytochrome P450, subfamily 7B, polypeptide 1^a

Laminin beta-2 chain precursor

Purigenic receptor P2Y, G-protein coupled 2

Aldose reductase-like protein

Cytokine-induced neutrophil chemoattractant-2^a

MAP-kinase phosphatase (cpg21)

RAB27B, member RAS oncogene family

Apoptotic death agonist BID

EGL nine homolog 3 (C. elegans)

Matrix metalloproteinase 3

Rat VL30 element ^a

Arginosuccinate synthetase

Endothelial cell-specific molecule 1

Matrix metalloproteinase 9^a

Receptor-interacting serine-threonine kinase 2

ATP-binding cassette, sub-family G (WHITE), member 1

Epiregulin precursor

Matrix metalloproteinase 10

RelB

BCL2-related protein A1

Fatty acid binding protein 4

Matrix metalloproteinase 12

Retinol-binding protein 1

Beta-nerve growth factor

F-box protein Fbx5

• Matrix metalloproteinase 13

• Ring finger protein 28

Bloom's syndrome protein homolog (mBLM)

Fibroblast growth factor 9

Mesothelin

Schlafen 4

Bone morphogenetic protein 2^a

Follistatin

Microtubule-associated protein

Small inducible cytokine A2

Bone morphogenetic protein 6^a

Fos-like antigen 1

6 ^a

Smhs1 protein

Bone morphogenetic protein 7

Gardner-Rasheed feline sarcoma viral (Fgr) oncogen homolog

Mitochondrial solute carrier protein

Solute carrier family 1, member 1

Brain-specific angiogenesis inhibitor 1-associated protein 2

GATA-binding protein 2

Myotubularin related protein 7

Solute carrier family 1, member 3

CCL3 (Mip-1a)

Gro

NADH-ubiquinone oxidoreductase MLRQ subunit

Solute carrier family 7 (cationic amino acid transporter, y⁺ system), member 1

CCL5 (RANTES)

Growth arrest specific 7

Neurofilament, heavy polypeptide

Solute carrier family 11, member 2

CCL7 (MCP-3)

GTP cyclohydrolase 1

Neuron specific protein PEP-19 (Purkinje cell protein 4)

Solute carrier family 20 (phosphate transporter), member 1

CD44 antigen

High mobility group AT-hook 1

Neuropeptide Y

Superoxide dismutase 2

Chemokine receptor (LCR1)^a

IκB-α

Neurospin precursor

T-cell death associated gene

Cholesterol 15-hydroxylase

IκB-β (nuclear factor kappa B p105 subunit)

Nitric oxide synthase 2

Testis-specific protein Bs13

Claudin-3

Inhibin beta-A

Organic cation transporter OCTN1

Tissue factor pathway inhibitor 2

Colony stimulating factor 2 (granulocyte-macrophage)

Inhibitor of apoptosis protein 1

Parvalbumin

Tissue-type transglutaminase

Colony stimulating factor 3

Insulin-like growth factor-binding protein 5

Phosphodiesterase 4B, cAMP-specific (dunce (Drosophila) homolog phosphodiesterase E₄)

Toll-like receptor 2

Complement factor B precursor (C3/C5 convertase)

Interleukin 1 alpha

Phospholipase A2, group 5

Tumor necrosis factor alpha-induced protein 2^a

CXCL1 (GROa)

Interleukin 1 beta

Phospholipase A2, group IIA (platelets, synovial fluid)

Tumor necrosis factor receptor superfamily member 5 precursor

CXCL2 (GROb)

Interleukin 6 (interferon, beta 2)

Phospholipid scramblase 1

Tumor necrosis factor receptor superfamily member 9 precursor

CXCL5 (LIX)

Interleukin 23, alpha subunit p19

Plasminogen activator inhibitor 2 type A

Uridine phosphorylase

CXCL10 (IP-10)

• Interleukin 13 receptor, alpha 2

Plasminogen activator, tissue