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Table 5 Genes whose expression showed the greatest change following incubation of chondrocytes with elevated IL-1β alone: genes of known function whose expression was most affected by IL-1 alone

From: Exogenous glucosamine globally protects chondrocytes from the arthritogenic effects of IL-1β

Genes whose RNA levels were reduced >80% relative to untreated control cultures
• A disintegrin and metaloproteinase domain 33 • c-fos-induced growth factor (vascular endothelial growth factor D) Kruppel associated box zinc finger 1 • Smoothelin
• Actin alpha 1 • Collagen type II alpha 1, chain precursor • Microfibril-associated glycoprotein precursor Solute carrier family 39 (iron-regulated transporter), member 1
Aggrecan 1 Crystallin, alpha B • Midline 1 • Sphingomyelin phosphodiesterase 3, neutral
• Ankyrin-like repeat protein Distal-less homeobox • Mitochondrial ribosomal protein L53 • Transforming growth factor, beta 2
• Annexin III (Lipocortin III) • DVS27-related protein Myocilin precursor • Zinc finger protein SLUG (neutral crest transcription factor Slug)
Cadherin-8 precursor • Dynein, cytoplasmic, intermediate chain 1 • NAD-dependent 15-hydroxyprostaglandin dehydrogenase  
Calpain 6 • Enolase 3, beta Osteomodulin (osteoadherin)  
• Carbonic anhydrase 3 Four and a half LIM domains 1 • Palmdelphina  
Cartilage link protein 1 a • Heat shock protein HSP 90 betaa • Phosphoribosyl pyrophosphate synthestase 2  
• Caveolin 3 Insulin-like growth factor binding protein 4 precursor • Programmed cell death protein 7  
Genes whose RNA levels were increased more than fivefold relative to untreated controls
Adaptor protein with pleckstrin homology and src homology 2 domains Cyclooxygenase-2 Keratin, type II cryosqueletal 8 Plasminogen activator, urokinase receptor
Adenosine A2B receptor Cytochrome P450, family 26, subfamily b, polypeptide 1 Lactose operon repressor Prostaglandin E synthase a
Adenosine monophosphate deaminase 3 Cytochrome P450, subfamily 7B, polypeptide 1a Laminin beta-2 chain precursor Purigenic receptor P2Y, G-protein coupled 2
Aldose reductase-like protein Cytokine-induced neutrophil chemoattractant-2a MAP-kinase phosphatase (cpg21) RAB27B, member RAS oncogene family
Apoptotic death agonist BID EGL nine homolog 3 (C. elegans) Matrix metalloproteinase 3 Rat VL30 element a
Arginosuccinate synthetase Endothelial cell-specific molecule 1 Matrix metalloproteinase 9a Receptor-interacting serine-threonine kinase 2
ATP-binding cassette, sub-family G (WHITE), member 1 Epiregulin precursor Matrix metalloproteinase 10 RelB
BCL2-related protein A1 Fatty acid binding protein 4 Matrix metalloproteinase 12 Retinol-binding protein 1
Beta-nerve growth factor F-box protein Fbx5 • Matrix metalloproteinase 13 • Ring finger protein 28
Bloom's syndrome protein homolog (mBLM) Fibroblast growth factor 9 Mesothelin Schlafen 4
Bone morphogenetic protein 2a Follistatin Microtubule-associated protein Small inducible cytokine A2
Bone morphogenetic protein 6a Fos-like antigen 1 6 a Smhs1 protein
Bone morphogenetic protein 7 Gardner-Rasheed feline sarcoma viral (Fgr) oncogen homolog Mitochondrial solute carrier protein Solute carrier family 1, member 1
Brain-specific angiogenesis inhibitor 1-associated protein 2 GATA-binding protein 2 Myotubularin related protein 7 Solute carrier family 1, member 3
CCL3 (Mip-1a) Gro NADH-ubiquinone oxidoreductase MLRQ subunit Solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
CCL5 (RANTES) Growth arrest specific 7 Neurofilament, heavy polypeptide Solute carrier family 11, member 2
CCL7 (MCP-3) GTP cyclohydrolase 1 Neuron specific protein PEP-19 (Purkinje cell protein 4) Solute carrier family 20 (phosphate transporter), member 1
CD44 antigen High mobility group AT-hook 1 Neuropeptide Y Superoxide dismutase 2
Chemokine receptor (LCR1)a IκB-α Neurospin precursor T-cell death associated gene
Cholesterol 15-hydroxylase IκB-β (nuclear factor kappa B p105 subunit) Nitric oxide synthase 2 Testis-specific protein Bs13
Claudin-3 Inhibin beta-A Organic cation transporter OCTN1 Tissue factor pathway inhibitor 2
Colony stimulating factor 2 (granulocyte-macrophage) Inhibitor of apoptosis protein 1 Parvalbumin Tissue-type transglutaminase
Colony stimulating factor 3 Insulin-like growth factor-binding protein 5 Phosphodiesterase 4B, cAMP-specific (dunce (Drosophila) homolog phosphodiesterase E4) Toll-like receptor 2
Complement factor B precursor (C3/C5 convertase) Interleukin 1 alpha Phospholipase A2, group 5 Tumor necrosis factor alpha-induced protein 2a
CXCL1 (GROa) Interleukin 1 beta Phospholipase A2, group IIA (platelets, synovial fluid) Tumor necrosis factor receptor superfamily member 5 precursor
CXCL2 (GROb) Interleukin 6 (interferon, beta 2) Phospholipid scramblase 1 Tumor necrosis factor receptor superfamily member 9 precursor
CXCL5 (LIX) Interleukin 23, alpha subunit p19 Plasminogen activator inhibitor 2 type A Uridine phosphorylase
CXCL10 (IP-10) • Interleukin 13 receptor, alpha 2 Plasminogen activator, tissue  
  1. aRepresents multiple probe sets. Bold circles indicate genes for which the IL-1-mediated effects on transcription were not inhibited by exogenous glucosamine (see text).