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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approach

Figure 2

Citrullination of F-actin capping protein α-1 subunit (CapZα-1) in synovial tissues of patients with rheumatoid arthritis. The extracts from synovial tissues from three patients (RA103, 107, and 109) were separated by two-dimensional electrophoresis. Proteins were stained with SYPRO Ruby protein gel stain (left column). After transfer, citrullinated proteins were detected by Western blotting with anti-modified citrulline (anti-MC) antibodies (middle column). The protein spots of CapZα-1 confirmed by Western blotting with anti-CapZα-1 antibodies (right column) are indicated by arrows.

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Arthritis Research & Therapy

ISSN: 1478-6362

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