Figure 6
Effects of antisera neutralising different cytokines on calcium 45 (45Ca) release from neonatal mouse calvarial bones stimulated by synovial fluids (SFs) from patients with osteoarthritis (OA) or with a loose prosthesis. (a) Effect of antiserum neutralising IL-1α on 45Ca release induced by SFs from patients with OA. (b) Effect of antiserum neutralising IL-1α on 45Ca release induced by SFs from patients with a loose prosthesis. (c) Effect of antiserum neutralising IL-1β on 45Ca release induced by SFs from patients with OA. (d) Effect of antiserum neutralising IL-1β on 45Ca release induced by SFs from patients with a loose prosthesis. (e) Effect of antiserum neutralising TNF-α on 45Ca release induced by SFs from patients with OA. (f) Effect of antiserum neutralising TNF-α on 45Ca release induced by SFs from patients with a loose prosthesis. (g) Effect of antiserum neutralising IL-6sR on 45Ca release induced by SFs from patients with OA. (h) Effect of antiserum neutralising IL-6sR on 45Ca release induced by SFs from patients with a loose prosthesis. (i) Effect of antiserum neutralising IL-17 on 45Ca release induced by SFs from patients with OA. (j) Effect of antiserum neutralising IL-17 on 45Ca release induced by SFs from patients with a+ loose prosthesis. In each experiment, five or six calvarial bones were incubated for 96 hours with SF (10%) from one patient and five or six bones with the same SF and different antisera. At the end of the experiment (96 hours), 45Ca release was analysed and compared to that in unstimulated control bones (100%). IL, interleukin; TNF-α, tumour necrosis factor-alpha.