Figure 2

Genomic organization, alternative splicing, and protein sequence of c19orf10. (a) The chromosomal localization of the region containing the c19orf10 gene is indicated on the ideogram. The area containing the c19orf10 gene is expanded and the base-pair positions are indicated. The location of a microsatellite marker linked to juvenile rheumatoid arthritis, D19S216, is also indicated. (b) The expanded region of chromosome 19 containing the c19orf10 gene. Three putative splicing variants are indicated (c19orf10.a, c19orf10.b, and c19orf10.c). Thick blocks indicate translated exons, open blocks indicate untranslated exons, horizontal lines indicate introns, and the arrows indicate the direction of transcription. (c) Alignment of protein products of c19orf10 splicing variants. Variants a and b seem to be complete sequences starting with an N-terminal methionine. Variant c does not start with an N-terminal methionine and is probably incomplete at the N-terminus. Lines above the sequence map the exons to the protein sequence. Shaded sequences indicate peptides observed by mass spectrometry. The putative N-terminal signal peptide is underlined with a solid black line. C63 and 92 are indicated by rectangles. c19orf10, chromosome 19 open reading frame 10.